3F of Outpatient Building
3F of Outpatient Building, including basic research room, pathological research room, genetic and metabolic disease research room, blood disease research room, kidney disease research room, ICU research room and skin disease research room. The basic research room has clinical gene amplification testing laboratory, molecular biology laboratory, virus serologic testing laboratory, pathology laboratory, cell culture laboratory and SPF animal laboratory. Pediatric Research Institute has good research conditions and relatively stable and cohesive technical force and scientific researchers. With most researchers holding master's and doctor's degrees, the Institute is a comprehensive pediatric research base closely combined with clinical medicine and a post-doctoral scientific research workstation in the realm of pediatric research in Hunan Province.
As a comprehensive experimental platform with a combination of scientific research and clinical experiments, Pediatric Research Institute undertakes scientific research and graduate education as well as many special clinical testing. The services cover various testing of flow cytometry, DNA and RNA testing of various pathogens, T cells spot test of Tuberculosis (TB), quantitative detection of bacterial endotoxin, relevant test of fungal infection diagnosis, testing of antigen antibodies of viruses such as respiratory viruses and enterovirus, chromosome conventional banding technique and high resolution banding technique, bone marrow morphology analysis, special staining of bone marrow and enzyme histochemical staining, testing of hemolytic anemia such as thalassemia, blood disease tests such as automatic electrophoresis analysis of hemoglobin; inhale and food allergen fluorescence immune detection specific IgE screening (Phad, fx5E) and 43 kinds of inhale, food and other chemical allergen single specific IgE detection and total IgE, eosinophilic cationic protein (ECP) testing, etc., as well as related antibody detection for autoimmune disease in children and kidney detection with immunofluorescent technique. The Institute can undertake research subjects such as cell culture and animal experiments.
In clinicopathologic diagnosis, we can diagnose rare diseases such as mastocytosis, pulmonary hemosiderosis and glycogen storage disease in children with the help of special staining such as Giemsa staining, iron staining and PAS staining. We can also diagnose and identify various kinds of refractory diseases such as tissue hyperplasia, and malignant tumors such as hematopoietic system tumor, neuroblastoma, rhabdomyoma and malignant germ cell tumors through immunohistochemical techniques. Using fine needle aspiration cytology, we can preliminarily identify tumor lesions and inflammatory lesions; we can also diagnose special infections such as tuberculosis and fungal infection with the help of special staining such as acid staining and PAS staining. We can also perform pathological cytology testing and screening of tumor cells and inflammatory cells in sputum, chest water and alveolar lavage, and differential counting of inflammatory cells to help clinical diagnosis.
Pediatric Research Institute was established on June 1, 2003 and was approved as a "post-doctoral scientific research workstation" by the Ministry of Personnel in 2006. As a pediatric research and postgraduate training base, Pediatric Research Institute has good research conditions and relatively stable and cohesive technical force and scientific researcher, as well as modern facilities. At present, the Institute mainly performs research on sepsis and pancreas injury and other critical diseases, as well as common virus infection in children, hereditary diseases and genetic pharmacology in children, blood diseases, kidney diseases and skin diseases in children.
With "being rigorous, careful, scientific and seeking the truth" as its operation principle, the Institute offers sincere services to patients in the hospital spirit of “solidarity, progress, innovation and self-transcendence”. The Institute has 22 medical workers, of whom 6 have senior titles, 7 have intermediate titles and 9 have junior titles. Three hold doctor's degree and six hold master's degree.
Li Liping, the academic leader of the Institute, female, is a chief analyst and director of Pediatric Research Institute, Hunan Children's Hospital. She is also deputy director of Assay Committee, Hunan Medical Association, member of Chinese Medical Doctor Association of Lab Medicine, member of Assay Committee, Chinese Medical Doctor Association, and managing director of Hunan Association for Laboratory Animal Sciences. Her area of specialization is molecular diagnostics, clinical immunology and immune assay, clinical virology and diagnosis. She once participated in the national natural fund project and multiple research projects of Hunan Department of Science and Technology and the Department of Health. At present, she is in charge of four research projects of Hunan Department of Science and Technology and the Department of Health, with the major research area of cytomegalovirus in human. In recent years, she has published more than 30 academic papers on all levels of academic conferences and various professional journals. "Research on the Correlation between cytomegalovirus-related genes and pathogenicity in infants and young children" won 2nd prize of 12th medical science and technology award of Hunan Province.
Leading technical strength
The Institute can perform cellular immune by means of flow cytometry, detection of antigen antibodies such as respiratory viruses and intestinal viruses based on fluorescence immunoassay, DNA and RNA testing of various pathogens by means of PCR and detection of Mediterranean anemia gene mutations. We have high resolution chromosome banding technique, perform inhale and food allergen detection and can undertake the research projects of cell culture and animal experiments. In terms of pathological detection, the Institute can perform lymphoma classification and confirmation, differential diagnosis of difficult diseases in children, cancer gene detection and malignant tumor prognosis evaluation, etc.
The Institute has a number of modern equipment, including FACSCantoTM Ⅱ 3-laser 10-color flow cytometer produced by Becton, Dickinson and Company, ABI7500 and Roche 4800 fluorescence quantitative PCR, ABI Veriti and MJPTC-200 qualitative gene PCR, ultramicro ultraviolet spectrophotometer, ultrasonic processing system, ultrapure water system, gel imaging system and electrophoresis system for molecular biology research; import inverted microscope, three gas CO2 incubator and ultra-low temperature freezer for cell culture; IVC for SPF animal experiments; imported multi-functional graphic analysis system for morphology analysis; freezing microtome and fluorescence microscope, pathologic samples ultrasonic fast processor and multi-observer microscope for pathology study; imported automatic allergens detector for allergy research; and import fully-automatic protein electrophoresis apparatus for serum, hemoglobin and cerebrospinal fluid protein analysis, as well as Leica chromosome automatic scanning and analysis system for chromosome analysis.
Strong research capability
The Institute has undertaken more than 80 research projects of National Nature Science Foundation of China, National Science Fund for Distinguished Young Scholars, provincial department level and hospital level. We have published more than 200 academic papers on various core journals and ten odd ones have been included in SCI. Genetic research laboratory of the Institute first discovered HGD pathogenic mutations of the genetic metabolic disease "alcaptonuria" in Chinese people and that 5 q14q21 chromosome deficiency is related to epileptic sudden death; we have identified a new congenital malformation syndrome of ulna and radius integration through genotype and phenotype analyses and found a Schmid critical metaphysis cartilage dysplasia caused by COLIOAI genetic mutation, which was reported to be the first mutation the causes Typex collagen growth. We have also found more than 100 rare karyotypes. These findings are of great importance for the research on human genetic diseases.